Two solutions, A and B, are made. Solution A contains Luminol in an alkaline environment, whilst solution B contains Potassium Ferricyanide in a 0,03% Hydrogen Peroxide solution. When the two solutions are mixed, they reacts and emit blue light
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Chemiluminescence of luminol. Two solutions are combined, the first solution contains luminol and sodium hydroxide. The second solution is 0.03% Hydrogen peroxide and potassium ferricyanide. The luminol and the hydrogen peroxide react to cause the chemiluminescence. The iron in potassium ferricyanide is a catalyst for the reaction. This is also used by crime scene investigators. A solution of hydrogen peroxide and luminol is placed into a spray bottle. When the solution comes into contact with blood, the iron present in hemoglobin will catalyze the reaction and it will begin to glow. www.monkeyvsgravity.com If you enjoy this type of video, subscribe! Music: Kevin MacLeod
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What happens when you combine luminol and hydrogen peroxide? In this video, UW Madison graduate, Jay Sekhon brightens, enlightens, and illuminates our knowledge of a phenomenon known as chemiluminescence. Special thanks to the guys and gals of Alpha Chi Sigma for spreading the chemistry word!
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Two solutions are made, solution "A" composed of 5 grams of sodium hydroxide in water and 0.1 grams of luminol. Solution "B" composed of 3% hydrogen peroxide and 0.25 grams of sodium ferrocyanide. When the two solutions are mixed produces a faint blue glow. Then 10 grams potassium ferricyanide is added to the combination to produce an intense blue glow that only lasts for about 20 seconds.
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Dr Hal Sosabowski and Dave "Soup" Campbell in action at a recent Royal Society of Chemistry meeting in Southampton
3-Nitrophthalhydrazide, prepared in a previous video, is reduced with a readily available reducing agent (thiourea dioxide, TUD) to luminol, which is obtained in 66% yield due to some minor complications with workup. A significantly higher yield would normally be expected based on a smaller batch run previously. The product is then used for two demonstrations of chemiluminescence: Potassium ferricyanide catalyzed light emission in water utilizing hydrogen peroxide and uncatalyzed light emission with NaOH in dimethyl sulfoxide using atmospheric oxygen. Note that the reaction mechanism is not given for the reduction. This is because I am led to believe that TUD functions by a single electron transfer mechanism, which is fairly confusing to draw out. The intro music clip is from "Stealing Fat" by The Dust Brothers. I believe this falls under fair use.
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Eric and Romil making magic happen in the lab...
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A simple demonstration of chemiluminescence or exogenous reaction. This is an excellent demonstration to get the attention of the students, they appreciate this demonstration very much that has been my experience all along.
The chemicals involved in this experiment are water (room temperature), Luminol, Sodium Perborate, and a 50% bleach solution. Luminol acts as the "light bulb," the Sodium Perborate and the 50% bleach solution act as the "battery," and the water acts mainly as the medium for the reaction to take place.
A suspension of White Phosphorus and Mineral Oil in Carbon Disulfide is made. Some drops of this suspension is placed on a piece of paper, causing the Carbon Disulfide to evaporate. This exposes the White Phosphorus to atmospheric Oxygen. As the White Phosphorus is covered by a thin layer of Mineral Oil, this modifies its reaction with Oxygen, making chemiluminescence in the gas phase clearly visible.
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UW-Madison Chemistry Prof. Bassam Z. Shakhashiri does a science demonstraiton during the 39th annual "Once Upon a Christmas Cheery, In the Lab of Shakhashiri" in December of 2008. Bassam ignites a mixture of nitrous oxide gas and carbon disulfide vapor in a demo from his newest book, "Chemical Demonstrations: A Handbook for Teachers of Chemistry, Volume 5," available now from the University of Wisconsin Press. See www.scifun.org for more information. A DVD of this program can be purchased at Educational Innovations: www.teachersource.com
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Chemiluminescence of luminol © Qc-experiment.qc.cx
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Chemiluminescence, Yay! The oxidant is hydrogen peroxide and the fuel a peroxyoxylate ester. The reaction is started in the dark with the liquid oxidant (H2O2 dissolved in an organic solvent) already in the glass flask and then the liquid fuel is added quickly from above. These reactants are mixed together by gently swirling. The reaction's light emission reaches a maximum after about 10 seconds. If you look carefully you can see the chemist's hand as he manipulates the reaction vessel. Like most chemiluminescent reactions, none of the energy released by the reaction ends up as heat: The temperature of this reaction mixture is almost exactly the same as the temperature of the reagents before mixing. Video from: www.shsu.edu
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Instead of the traditional potassium ferricyanide catalyst I used copper as a catalyst with luminol. 1Liter Luminol solution 0.2g Luminol 0.5g CuSO4 0.5g Na2CO3 0.5g (NH4)2CO3 21g NaHCO3 Oxidizer 0.1% H2O2
Solution A (right)- 5-amino-1,4-phthalazdione (luminol) mixed with 10mL of 3M NaOH solution & 90mL of deionized water. Solution B (left)- 4mL of 3% aqueous potassium ferricyanide, 20mL of 3% hydrogen peroxide, and 160mL of deionized water. Add Solution A and Solution B together simultaneously and observe the Chemiluminescence.
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Chemiluminescence Demonstration to Westlake Middle School students by the Sarpong Group at Berkeley
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The ability to multiplex fluorescent labels has greatly improved the utility of applications like Western blotting. The simultaneous detection of 2 or more protein targets provides both cost and time-savings increasing lab productivity. While fluorescence imaging is rapidly advancing, chemiluminescent Western blot analysis still remains the most sensitive method for detection of low-copy protein targets. Although most loading control proteins are highly expressed proteins that can be easily detected using fluorescent labels, most targets of interest are low-copy number proteins that demand the greatest potential detection sensitivity. To address this need we have developed a method that accommodates simultaneous incubation of both fluorescent and chemiluminescent labeled secondary antibodies. This webinar will outline this approach and present data to support the validity and reliability of the method. Signal output enhancers and post image processing for presentation and analysis will also be discussed.
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Two of my last small blobs were spent on making this video. So there you have it. A closeup of one of the scariest substances out there. It fumes, it glows, it burst into flames, though the glow can't be appreciated in this video, so here's a more natural looking photo: www.flickr.com (it's not phosphorescence, it's chemiluminescence) I've put another version at Wikipedia. Now there's finally a hi-res photo of WP glowing on the Web. I won't say how I've made it. It is way too dangerous, and lots of stupid kids could get hurt. I don't want that, and I'm furious when I see dumb kids doing careless shit. You can actually DIE if you mess with this. Head of a needle can kill you, and if it doesn't kill you, it wrecks your liver and bones. I'm actually very afraid of WP. It's pretty, but the strained atoms in its P4 molecules just want to get hold of anything out there, and they do it very well. It's best if it's kept as an element sample in a small vial of water. Video contains 3 scenes: fuming and glowing, spontaneous combustion and reaction with chlorine. At first it was disabled, because someone clearly thinks it's not cool to use a remix for the soundtrack of this educational video. Now there's a scary soundtrack from one old movie which is very... ontopic. The movie is "The Young Poisoner's Handbook" (performer unknown, it's not even listed in the soundtrack list), and the music is called "March from 'Funeral Music for Queen Mary'", written by famous composer Henry <b>...</b>
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Montage pour commémorer l'année internationnale de la chimie ( AIC ) Setup made to commemorate the Internationnal year of chemistry ( IYC * AIC in french ) Thanks to Nurdrage for the info and "how to " www.youtube.com A must for all chemistry lover out there!!! Music: NIN The big come down
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.Chemiluminescence Experiment in my Organic Chemistry Class. This was our final product.
When a small spatula of red phosphorus is heated in a test tube, it will decompose and the other allotrope, white phosphorus, is formed. White phosphorus vapour will emit green light in the vapour phase, when in contact with moist air. This is due to oxidation of the phosphorus, in wich the intermediates (PO)2 and HPO will be in the excited state (and thus emit light). When white phosphorus is oxidised, various oxides are formed including P4O6 and P4O10. After only a short time of heating, quite a lot of white phosphorus has condensed in the upper parts of the test tube, as seen in the video. While white phosporus self-ignites in air at 30C, the phosphorus in the test tube does not ignite, probably because the oxygen level is the test tube is too low (consumed in the reaction with white P vapour), and the diffusion of air in the test-tube is also a slow process. However, should the test-tube break, the result would probably be a fire. Although the amount of phosphorus used in this experiment is very small (100mg or so), it still introduces a risk, as white phosphorus is extremely toxic (LD50 50-100mg), and the burning material on the skin can cause severe burns. This experiment should be done only with good ventilation, preferably outside or in a fume hood (I did it in a fume hood). Wear goggles and gloves.
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( www.abnova.com ) - Photon™ uses a state-of-the-art, photon-counting multiplier tube as the detector element to detect very small amounts of analytes, in the range of attomoles, by counting the number of photons generated from the chemiluminescent or bioluminescent experiment. More videos at Abnova http
Explain and view chemiluminsecence. Observe how luminol is converted to an excited state in the presence of an oxidizer such as hydrogen peroxide. As the excited state molecule decays or returns to the ground state energy is produced as light
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A chemical reaction producing glowing paper. More at madphysics.com
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The Odyssey Fc Imager is a unique CCD imager that offers excellent infrared (IR) fluorescent and chemiluminescent imaging performance in one system. At the push of a button, targets of interest can be imaged over more than six logs of linear dynamic range in every acquisition. Quantitative IR Western blots have never been easier. Chemiluminescence detection is simplified with the Odyssey Fc Imaging System. Go greener with the Odyssey Fc -- eliminate film and darkroom steps, along with the hazardous waste and excessive water usage of film development. bit.ly
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Luminol Preparation. The solution shows chemiluminescence(glows in dark), once the Solution A and B are mixed and Potassium Ferrocyanate is added..
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Mixtures of Luminol and Hydrogen peroxide in the presence of NaOH base produced a light emitting reaction called chemiluminescence.
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Dr. Arnab Bhattacharya (TIFR) demonstrates the Traffic-lights and TCPO-Chemiluminescence reactions at The Fergusson College Chemistry Club, Pune. Traffic lights reaction : Pour about 150 mL of 0.1 M dextrose into a bottle. Add 6 mL of 3 M NaOH solution and 4-5 mL of indigo carmine solution. This should be done about 30 minutes before the demonstration is to be used. The color of the solution will gradually change and become yellow. When the solution is shaken gently it will turn back to green or a light blue. This cycle can be repeated many times. After a while the indicator seems to "wear out". When this happens add more indigo carmine to enhance the color changes. TCPO-Chemiluminescence : mix together - 10mL Diethyl Phthalate (solvent) 3mg of fluorescent dye (9,10-bis(phenylethynyl)anthracene for green, Rubrene for yellow, 9,10-diphenylanthracene for blue, and rhodamine B for red.) 50mg TCPO (Bis-2,4,6-(trichlorophenyl)oxalate) 100mg sodium acetate 3mL 30% hydrogen peroxide (add last to start reaction)
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This is a reaction conducted by students in an organic chemistry lab at Christopher Newport University. The reactants, Luminol (5-aminophthalhydrazide) and 3-aminophthalate (excited state), are poured down a funneled tube to create 3-aminophthalate (ground state) which gives off photons as a by-product.
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This demonstration shows that the energy of a chemical reaction can be given out as light as well as heat. However the demonstration could be performed simply to stimulate interest in chemistry or at an open day or other public demonstrations.
