Chemiluminescence


Chemiluminescence, Yay! The oxidant is hydrogen peroxide and the fuel a peroxyoxylate ester. The reaction is started in the dark with the liquid oxidant (H2O2 dissolved in an organic solvent) already in the glass flask and then the liquid fuel is added quickly from above. These reactants are mixed together by gently swirling. The reaction's light emission reaches a maximum after about 10 seconds. If you look carefully you can see the chemist's hand as he manipulates the reaction vessel. Like most chemiluminescent reactions, none of the energy released by the reaction ends up as heat: The temperature of this reaction mixture is almost exactly the same as the temperature of the reagents before mixing. Video from: www.shsu.edu


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Chemiluminescent paper (Mad Physics)


A chemical reaction producing glowing paper. More at madphysics.com


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Chemiluminescent Elephant Toothpaste


Kramer, Daniel and Kelton describe a chemiluminescent elephant toothpaste reaction.


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Elementary Productions: Chemiluminescence of Luminol


Two solutions, A and B, are made. Solution A contains Luminol in an alkaline environment, whilst solution B contains Potassium Ferricyanide in a 0,03% Hydrogen Peroxide solution. When the two solutions are mixed, they reacts and emit blue light


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Chemiluminescence


Chemiluminescence of luminol © Qc-experiment.qc.cx


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Photon™ - 96 well Chemiluminescence Reader


( www.abnova.com ) - Photon™ uses a state-of-the-art, photon-counting multiplier tube as the detector element to detect very small amounts of analytes, in the range of attomoles, by counting the number of photons generated from the chemiluminescent or bioluminescent experiment. More videos at Abnova http


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Infrared Fluorescence and Chemiluminescence for Western Blots in One System


The Odyssey Fc Imager is a unique CCD imager that offers excellent infrared (IR) fluorescent and chemiluminescent imaging performance in one system. At the push of a button, targets of interest can be imaged over more than six logs of linear dynamic range in every acquisition. Quantitative IR Western blots have never been easier. Chemiluminescence detection is simplified with the Odyssey Fc Imaging System. Go greener with the Odyssey Fc -- eliminate film and darkroom steps, along with the hazardous waste and excessive water usage of film development.


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Chembows/Bio - Chemical Chemiluminescent Skyscape "Sicktoria" BC 'Canada' 6:55pm Sept 4th/'11


Globalist Military Bio Chemical Warfare/chemiluminescent skies in "Sicktoria", BC, Canada causing severe headaches, eye, ear, nose, throat respiratory, cardiovascular, central nervous system, digestive system disorders ... gasping for breath with blocked/runny nose, ears and eyes itching/burning/leaking, extremely sore throat - spots on uvula and skin on back of throat, headache - frontal lobe, intra cranial pressure, dizziness/vertigo, burning feeling in head/neck lungs... this is a little different from the last aerosolised chemical/biological mix which chokes us with a gooey/gluey polymer substance which is next to impossible to get out of the lungs (not surprisingly pneumonia symptoms are common with that mix).


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Chembows/Bio - Chemical Chemiluminescent Skyscape "Sicktoria" BC 'Canada' 7:06pm Sept 4th/'11


Globalist Military Bio Chemical Warfare/chemiluminescent skies in "Sicktoria", BC, Canada causing severe headaches, eye, ear, nose, throat respiratory, cardiovascular, central nervous system, digestive system disorders ... gasping for breath with blocked/runny nose, ears and eyes itching/burning/leaking, extremely sore throat - spots on uvula and skin on back of throat, headache - frontal lobe, intra cranial pressure, dizziness/vertigo, burning feeling in head/neck lungs... this is a little different from the last aerosolised chemical/biological mix which chokes us with a gooey/gluey polymer substance which is next to impossible to get out of the lungs (not surprisingly pneumonia symptoms are common with that mix).


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Chembows/Bio - Chemical Chemiluminescent Skyscape "Sicktoria" BC 'Canada' 7:05pm Sept 4th/'11


Globalist Military Bio - Chemical Warfare/Skyscape in "Sicktoria", BC, Canada causing severe headaches, eye, ear, nose, throat respiratory, allergies, cardiovascular, degenerative central nervous system and digestive system disorders, neck glands are EXTREMELY sore/swollen, shooting pains throughout the body, fever, chills, lethargy, malaise, aches, pains and a whole pile more really UGLY symptoms. WE CAN BARELY BREATHE AT TIMES AND WE KEEP BEING WOKEN UP BY FREQUENCY WHICH SEEMS TO DRY OUT OUR MOUTHS AND KILL OUR THROATS DOZENS OF TIMES A NIGHT...


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Amersham™ ECL™ Prime


www.gelifesciences.com Chemiluminescent Amersham™ ECL™ Prime is sensitive, stable, and precisely quantitative across a wide dynamic range of protein levels, and is highly efficient in its consumption of expensive antibody reagents. The improved performance of ECL Prime compared with other chemiluminescent reagents is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst. www.gelifesciences.com


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Make a Color Changing Glow Stick


We make a glow stick that changes colour with time. To do so, we prepare our standard chemiluminescent mixture: 10mL Diethyl Phthalate 3mg of 9,10-bis(phenylethynl)anthracene 5mg Rhodamine B 50mg TCPO 100mg sodium acetate 3mL 30% hydrogen peroxide (add last to start reaction) First the red Rhodamine B glows and masks the 9,10-bis(phenylethynl)anthracene. But since Rhodamine B is unstable it slowly breaks down and eventually the glow stick will turn green. The whole process takes about ten minutes.


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Elementary Productions: Gas phase chemiluminescence with P4


A suspension of White Phosphorus and Mineral Oil in Carbon Disulfide is made. Some drops of this suspension is placed on a piece of paper, causing the Carbon Disulfide to evaporate. This exposes the White Phosphorus to atmospheric Oxygen. As the White Phosphorus is covered by a thin layer of Mineral Oil, this modifies its reaction with Oxygen, making chemiluminescence in the gas phase clearly visible.


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LUMINOL (Chemiluminescence) glow stick


Two solutions are made, solution "A" composed of 5 grams of sodium hydroxide in water and 0.1 grams of luminol. Solution "B" composed of 3% hydrogen peroxide and 0.25 grams of sodium ferrocyanide. When the two solutions are mixed produces a faint blue glow. Then 10 grams potassium ferricyanide is added to the combination to produce an intense blue glow that only lasts for about 20 seconds.


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Learn to Drive by Chemiluminescence


Dr Hal Sosabowski and Dave "Soup" Campbell in action at a recent Royal Society of Chemistry meeting in Southampton


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Chemiluminescence Reaction using Luminol and Hydrogen Peroxide


Mixtures of Luminol and Hydrogen peroxide in the presence of NaOH base produced a light emitting reaction called chemiluminescence.


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Glowy Stuff


We weren't really sure how the glow stick reaction worked, and we were entirely too lazy to look it up... but since the PH of the glow stick solution tested low, we thought it might be fun to add a basic solution to it, to see what would happen. Turns out it catalyzes the chemiluminescent reaction, giving more light in less time!


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HUGE 2 Liter Glow stick!!!


A full two liters of glow fluid, about as much as you would find in 100 medium sized glow sticks. Imagine going to a rave and glowsticking with a couple of these! Not the world's biggest single glow stick, but still awesome. We made this using all the same chemicals in our previous glow stick making video just scaled up by a factor of 100. The link is here: www.youtube.com Since everyone keeps asking: NO you cannot drink it, it's highly toxic, it causes cancer, liver damage and destroys your kidneys, assuming you survive the initial poisoning. Our website at: sites.google.com


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Poison Skies Time Lapse SICKtoria, BC, Canada, late September, 2011


Globalist Military Bio Chemical Warfare/chemiluminescent skies in "Sicktoria", BC, Canada causing severe headaches, eye, ear, nose, throat respiratory, cardiovascular, central nervous system, digestive system disorders ... gasping for breath with blocked/runny nose, ears and eyes itching/burning/leaking, extremely sore throat - spots on uvula and skin on back of throat, headache - frontal lobe, intra cranial pressure, dizziness/vertigo, burning feeling in head/neck lungs... this is a little different from the last aerosolised chemical/biological mix which chokes us with a gooey/gluey polymer substance which is next to impossible to get out of the lungs (not surprisingly pneumonia symptoms are common with that mix).


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V 42 Chemiluminescence - Chemolumineszenz


More videos and detailed information on www.netexperimente.de If you are interested in more science stuff take a look on http If you like to know more about my work on science and education contact or follow me on facebook or twitter. Sven Sommer


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How to make TCPO (for making glow sticks)


How to make TCPO or bis(2,4,6-trichlorophenyl) oxalate, used in glow stick reactions. WARNING: This procedure should only be performed by, or under the direct supervision of, an experienced chemist. Please refer to the material safety data sheets of all chemicals for their hazards. Synthesis must be performed in a fumehood. Full written procedure can be found at our website: sites.google.com The exact same process is used in glow sticks. This is NOT mountain dew. Mountain dew cannot glow like this, it does not have the crucial TCPO chemical or any chemical like that. The reaction will glow in the dark for several minutes if you use ethyl acetate as the solvent. It will glow for several hours if you use diethyl phthalate as the solvent. Thanks for watching! Please subscribe, rate and comment! All the chemicals used in this video were purchased from Alfa Aesar. www.alfa.com


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Make a Complete Glow Stick - Refillable Too!


How to Make a Complete and Refillable Glow Stick with a steampunk style to it. The idea is simple, use compression fittings normally used in plumbing to create a glow stick tube that can be emptied and refilled when the fluid dies. The vials or ampoules of hydrogen peroxide is created from cheap glass pipettes that are flame sealed. The other chemicals can be found from our original glow stick reaction video: www.youtube.com An important change though is the use of diethyl phthalate solvent rather than ethyl acetate. Diethyl phthalate gives a longer lasting light. Other aliphatic dialkyl phthalates can be used including diethylhexyl phthalate so you can use whatever you have onhand. If built correctly and the purity of chemicals is good then it will last 3-8 hours. If you're having problems with leaking be sure to use teflon tape. Subscribe, rate and comment! Special note: Brass is pretty hard, so if you glowstring with this and hit someone... it's going to HURT... so be careful! Our website at: www.nurdrage.com


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Make Glow Sticks - The Science


We show how to make glow sticks and go through all the chemicals needed as well as how to make different colors. We also talk about the chemistry and scientifically research a proposed mechanism. To make the glow stick mix together the following: 10mL Diethyl Phthalate (solvent) 3mg of fluorescent dye (see below) 50mg TCPO (see below) 100mg sodium acetate 3mL 30% hydrogen peroxide (add last to start reaction) The fluorescent dye can be 9,10-bis(phenylethynyl)anthracene for green, Rubrene for yellow, 9,10-diphenylanthracene for blue, and rhodamine B for red. TCPO is expensive to buy but can be made for much cheaper by following the directions in our previous video: www.youtube.com The chemicals, including the ones to make TCPO were purchased from alfa aesar: www.alfa.com The Rhodamine B and Rubrene dyes were instead purchased from sigma Aldrich www.sigmaaldrich.com The dyes and TCPO are carcinogenic and gloves should be worn when handling them. The TCPO and sodium acetate amounts can be varied considerably and still obtain good light. Smaller quantities tend to be dimmer and decay faster, while larger quantities last longer. For those of you higher level individuals that actually do want to know the mechanism for this process, please refer to this article: pubs.acs.org


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NEW! Omniglow Stylite, the lowest-cost, light guided, illuminated endotracheal intubating stylet.


For 15 years OMNIGLOW has produced medical-grade chemiluminescent devices for both diagnostic and clinical use and is the world's largest producer of chemiluminescent devices for medical applications. For 2011, OmniGlow introduces our latest device, the patent-pending StyLite™ Illuminated Intubating Stylet. www.omnimedtech.com For use in Endotracheal Intubation Procedures, OmniGlow® StyLites™ are immediately available in 11Fr and 14Fr, with 7Fr coming soon. Emitting white light, they are the lowest priced Illuminated stylet available, providing 3 minutes of effective light. Individually packaged non-electric StyLites™ have no batteries, and can be easily bent into shape as needed. They are non-toxic, sterile, and latex free. www.omnimedtech.com


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Time lapse globalist HAARP Screen SICKtoria, BC, Canada


Globalist Military Bio Chemical Warfare/chemiluminescent skies in "Sicktoria", BC, Canada causing severe headaches, eye, ear, nose, throat respiratory, cardiovascular, central nervous system, digestive system disorders ... gasping for breath with blocked/runny nose, ears and eyes itching/burning/leaking, extremely sore throat - spots on uvula and skin on back of throat, headache - frontal lobe, intra cranial pressure, dizziness/vertigo, burning feeling in head/neck lungs... this is a little different from the last aerosolised chemical/biological mix which chokes us with a gooey/gluey polymer substance which is next to impossible to get out of the lungs (not surprisingly pneumonia symptoms are common with that mix).


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How to set up a Western (part 1) - The Ingredients


Introduction to setting up a Western Experiment from start to finish using Nupage, SureLock, iblot, Novex, Benchpro 4100 and the WesternBreeze(TM) kit. Learn more by searching for the product of interest at www.invitrogen.com


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How Electrochemiluminescence (ECL) Works


This video shows an animated representation about the working of eectrochemiluminescence. This video is freely available and created by Roche Professional Diagnostics. Electrochemiluminescence or electrogenerated chemiluminescence (ECL) is a kind of luminescence produced during electrochemical reactions in solutions. In electrogenerated chemiluminescence, electrochemically generated intermediates undergo a highly exergonic reaction to produce an electronically excited state that then emits light. ECL excitation is caused by energetic electron transfer (redox) reactions of electrogenerated species. Such luminescence excitation is a form of chemiluminescence where one/all reactants are produced electrochemically on the electrodes. ECL is usually observed during application of potential (several volts) to electrodes of electrochemical cell that contains solution of luminescent species (polycyclic aromatic hydrocarbons, metal complexes) in aprotic organic solvent (ECL composition).


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Immunoassay Analyzer -- Evidence Investigator by Randox


The No.1 choice for research, clinical, forensic and veterinary testing A sophisticated semi-automated biochip system perfectly designed for research, clinical, forensic and veterinary applications. Using the same multiplexing technology as the fully automated evidence®, Biochip Array Technology, this system offers many unique benefits. The Evidence Investigator has a wide test menu available including cardiac, cytokine, drugs of abuse, cerebral, thyroid, adhesion molecules, tumour monitoring, fertility, Ranplex CRC DNA array, growth promoter and anti-microbial arrays. In addition to the extensive range of existing analytes, over 220 new tests are in development. Further information available on www.randox.com . Benefits: One system for protein and DNA biochips. Multiplexing technology: one sample = multiple results. Extensive range of panels available with more in development. Minimal sample volume: 25-200µl per array (oral fluid). Ready to use biochips supplied with all reagents necessary. Multi-analyte calibrators, controls and reagents. DNA based biochips for detection of polymorphisms and mutations. A revolution in diagnostics, the Evidence Investigator has the capabilities to maximise your laboratory efficiency. Find out more at www.randox.com . Suggested new tags: ELISA, Chemiluminescent, analyzer, analyser, immunoassay, protein, DNA, biochips, clinical, diagnostics


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化學示範實驗:利用魯米諾偵測血液(Using the Luminol Test to Detect Blood)


詳細資料:highscope.ch.ntu.edu.tw Chemical Demonstrations: Using the Luminol Chemiluminescent Test to Detect Blood 魯米諾化學發光反應是刑事鑑定人員檢測血液在犯罪現場的殘留痕跡所用的方法之一。在這個示範實驗中,混合魯米諾、過氧化氫和氫氧化鉀在噴霧瓶中,噴灑魯米諾溶液在可能會有血液的地方。由於血液裡的血紅素含有鐵離子可當作催化劑,使魯米諾發光,因此當魯米諾噴灑在有血液的地方會放出藍色螢光。請按下面連結,詳見本示範實驗的示範步驟、藥品與器材、原理和概念、教學提示等。 highscope.ch.ntu.edu.tw 提供者:高瞻計畫中學教學資源平台國立台灣大學化學系highscope.ch.ntu.edu.tw


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Purdue Potions Class


This is a video of a lab in Chemistry 115 (potions class) where we prepared a chemiluminescent solution.


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